Course Description


This workshop provides practical approaches for the understating flow cytometry data, its analysis, Flow Cytometry Standard (FCS) format, and to produce visualizations for presentation and/or publication. During this two-day workshop, students are introduced to data management and quality control, processing of data with the package FlowJo, generate figures and statistical data for effectively communication. This workshop is tailored to researchers with limited flow cytometry experience, researchers doing less that 10-parameter cytometry, and experience researchers interested in becoming familiar with functional assay data, cytometry data model, and other areas of cytometry.  At the end of the course, you will be able to import flow cytometry data, evaluate the quality of the data, extract statistics, and produce clear figures. Participants would learn to use FlowJo with practical examples.

Course Outline

  • Import data to FlowJo and evaluate its quality
  • Compensation
  • Identify examples of good and bad data display.
  • Extract statistics
  • Produce clear figures
  • Analyze a simple immunostaining experiment
  • Analyze a Cell Cycle experiment
  • Analyze a Functional assay experiment
  • Analyze a Cytometry Bead Assays experiment
  • Effective and ineffective data visualization

Textbook Information

Software/equipment requirements


  • FlowJo v10. Students are expected to have a fully functional license. Trial license will not suffice.
  • Office suite with Word processor, Spreadsheet and Presentation program


Target Audience (if any)


People novice to Flow Cytometry or unfamiliar with its data analysis


General Training Rate:

Discounted Training Rate:
$815.00 - NIH Community (Trainees, Contractors, Employees, Tenants working at one of the NIH campuses)
$895.00 - Academia, US Government (Non-NIH), US Military

Technology Fee

Although no grades are given for courses, each participant will receive Continuing Education Units (CEUs) based on the number of contact hours. One CEU is equal to ten contact hours. Upon completion of this course each participant will receive a certificate, showing completion of the workshop and 1.4 CEUs.

Refund Policy
100% tuition refund for registrations cancelled 14 or more calendar days prior to the start of the workshop.

50% tuition refund for registrations cancelled between 4 to 13 calendar days prior to the start of the workshop.

No refund will be issued for registrations cancelled 3 calendar days or less prior to the start of the workshop.

All cancellations must be received in writing via email to Ms. Carline Coote at registrar@faes.org.

Cancellations received after 4:00 pm (ET) on business days or received on non-business days are time marked for the following business day.

All refund payments will be processed by the start of the initial workshop.



Familiarity with the basic principles of flow cytometry or IMMU 022

IMMU 022 | Flow Cytometry Experiment Design

To Register Click on "Add to Cart"
Section Title
Flow Cytometry Data Analysis and Visualization
W, Th
9:00AM to 5:00PM ET
Aug 10, 2022 to Aug 11, 2022
Schedule and Location
Contact Hours
Fee (Includes Tuition and Technology Fee)
Eligible Discounts Can Be Applied at Checkout $1,124.00
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