Live sessions on December 13, 15-17, 2021 from 9am to 5pm ET. No class on December 14, 2021.
Bulk transcriptomes have provided considerable insights and fostered the discovery and characterization of GRN (Gene Regulatory Network)s. However, bulk transcriptomes provide population-based averaged measurements which blur cell heterogeneity and developmental dynamics of asynchronous cell populations. Single-cell transcriptome technologies (scRNA-seq) capture cell heterogeneity and thus are useful for the discovery of cell populations, identification of cell mutants, and quantification of subpopulations. Leveraging on the ability of generating thousands of individual measurements from scRNA-seq, lots of methods have been developed to capture spatial or temporal information from cell populations. This course will introduce basic steps of the scRNA-seq processing, starting from fastq coming off 10x sequencer to spatial and temporal analysis using popular R packages. Then, we will discuss case studies that can be addressed using scRNA-seq.
Experience with R is required; experience with seq analysis in general is advantageous
Simultaneous access to two screens is highly recommended for adequate learning experience. Examples include two laptops, one computer with two screens, one laptop and one tablet, etc.
General Training Rate:
Discounted Training Rate:
$1,155.00 - NIH Community (Trainees, Contractors, Employees, Tenants working at one of the NIH campuses)
$1,285.00 - Academia, US Government (Non-NIH), US Military
Although no grades are given for courses, each participant will receive Continuing Education Units (CEUs) based on the number of contact hours. One CEU is equal to ten contact hours. Upon completion of this course each participant will receive a certificate, showing completion of the workshop and 2.8 CEUs.
100% tuition refund for registrations cancelled 14 or more calendar days prior to the start of the workshop.
50% tuition refund for registrations cancelled between 4 to 13 calendar days prior to the start of the workshop.
No refund will be issued for registrations cancelled 3 calendar days or less prior to the start of the workshop.
All cancellations must be received in writing via email to Ms. Carline Coote at firstname.lastname@example.org.
Cancellations received after 4:00 pm (ET) on business days or received on non-business days are time marked for the following business day.
All refund payments will be processed by the start of the initial workshop.