Course Description
Background
This five-day online workshop will utilize a combination of lectures and remote Q&A lab sessions in a “boot camp”-like approach for cell-biologists who seek to learn imaging techniques used extensively in biomedical research. This workshop will first begin with training in immunofluorescence and cell transfection with an early introduction to automated imaging and widefield fluorescence microscopy. The workshop will then fully focus on the world of confocal microscopy, a powerful and popular extension of fluorescence microscopy, allowing 3-dimensional localization and dynamics of cellular components. Through interactive online lectures anAd labs participants will be introduced to the most commonly used confocal microscopy techniques in addition to extensive training in image analysis using Fiji. Participants will also have interactive remote sessions with highly qualified scientists from Leica, Nikon, Olympus and Zeiss, where they will receive training in microscope basics and imaging techniques on each individual platform.
Lecture Topics
Introduction to Fluorescence Microscopy
Immunofluorescence (with workflows)
Cell Transfection for Fixed and Live Cell Imaging (with workflows)
High throughput Screening and Automated Imaging
Confocal Microscopy
Light sheet Microscopy
2-Photon Excitation;
TIRF
Introduction to Super-resolution Imaging (Airyscan, SIM, STED, SMLM)
Image Processing and Analysis using Fiji
Colocalization of Proteins
Spectral Unmixing
FRET
FRAP and FLIP
PhotoActivation
Remote Lab Topics
Colocalization using multi-color fluorescence imaging
Deconvolution
FRET
Spectral Unmixing
Protein Dynamics Using FRAP, FLIP, and PA-GFP
Image Processing and Analysis using Fiji
Who Should Attend?
Biomedical Scientists just entering the world of imaging and confocal microscopy.
Faculty Members & Instructors
Instructors are leading experts in the field from both academia and industry.
Credit
Although no grades are given for courses, each participant will receive Continuing Education Units (CEUs) based on the number of contact hours. One CEU is equal to ten contact hours. Upon completion of this course each participant will receive a certificate, showing completion of the workshop and 3.5 CEUs.
Refund Policy
100% tuition refund for registrations cancelled 14 or more calendar days prior to the start of the workshop.
50% tuition refund for registrations cancelled between 4 to 13 calendar days prior to the start of the workshop.
No refund will be issued for registrations cancelled 3 calendar days or less prior to the start of the workshop.
Notification
All cancellations must be received in writing via email to Ms. Carline Coote at registrar@faes.org.
Cancellations received after 4:00 pm (ET) on business days or received on non-business days are time marked for the following business day.
All refund payments will be processed by the start of the initial workshop.
