Genome engineering provides the ability to manipulate gene expression in a variety of cell types and animal models. This course will provide an overview of the current major genome engineering platforms and cover CRISPR genome editing technology in depth.
The first part of the course will focus on the general principles of genome editing technology including design, choice of format, delivery, efficiency, specificity, clonal isolation, genotyping, and validation. The second part of the course will address different applications including genome editing in mice, zebrafish, and induced pluripotent stem (iPS) cells, disease modeling, generation of reporter lines, and high throughput approaches. We will discuss strategies to make CRISPR gene editing more efficient, flexible, and specific. We will explore recent advances in the CRISPR field including base editors, prime editing, epigenome editing and microbial genome editing. We will also examine sequencing and quality control considerations for genome editing projects. Hands-on training on in-silico tools and carefully designed team projects will accompany the lecture material to provide practical training in design, assembly, transfection, and detection/evaluation steps of a typical genome editing workflow.
General Principles of Genome Engineering; DNA Repair Mechanisms; CRISPR-mediated Gene Modulation, Epigenome Editing, Targeted Integration, Genome Editing Workflow, Gene Correction in iPSCs; Targeted Mutagenesis in Zebrafish; CRISPR Genome Engineering in ES Cells and Embryos for Mouse Model Generation; Developing Reporter Lines; Sequencing and Quality Control Considerations.
Class size will be limited to 32
This workshop is suitable for researchers interested in integrating CRISPR technology into their research and/or for those who wish to acquire deeper understanding of genome editing techniques. Attendees are expected to have a working knowledge in molecular biology and/or have completed graduate level molecular biology courses for best learning experience.
This workshop is taught by subject matter experts from the National Institutes of Health and industry (MilliporeSigma).*
*This workshop is purely educational/training. FAES does not endorse any specific company/product; nor do not have any financial agreements/commission from MilliporeSigma.
Although no grades are given for courses, each participant will receive Continuing Education Units (CEUs) based on the number of contact hours. One CEU is equal to ten contact hours. Upon completion of this course each participant will receive a certificate, showing completion of the workshop and 3.5 CEUs.
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