Loading...

Course Description

Genome engineering provides the ability to manipulate gene expression in a variety of cell types and animal models. This course will provide an overview of the current major genome engineering platforms and cover CRISPR genome editing technology in depth.

The first part of the course will focus on the general principles of genome editing technology including design, choice of format, delivery, efficiency, specificity, clonal isolation, genotyping, and validation. The second part of the course will address different applications including genome editing in mice, zebrafish, and induced pluripotent stem (iPS) cells, disease modeling, generation of reporter lines, and high throughput approaches. We will discuss strategies to make CRISPR gene editing more efficient, flexible, and specific. We will explore recent advances in the CRISPR field including base editors, prime editing, epigenome editing and microbial genome editing. We will also examine sequencing and quality control considerations for genome editing projects. Hands-on training on in-silico tools and carefully designed team projects will accompany the lecture material to provide practical training in design, assembly, transfection, and detection/evaluation steps of a typical genome editing workflow.

Discussion Topics
General Principles of Genome Engineering; DNA Repair Mechanisms; CRISPR-mediated Gene Modulation, Epigenome Editing, Targeted Integration, Genome Editing Workflow, Gene Correction in iPSCs; Targeted Mutagenesis in Zebrafish; CRISPR Genome Engineering in ES Cells and Embryos for Mouse Model Generation; Developing Reporter Lines; Sequencing and Quality Control Considerations.

Class size will be limited to 32

Target Audience

This workshop is suitable for researchers interested in integrating CRISPR technology into their research and/or for those who wish to acquire deeper understanding of genome editing techniques. Attendees are expected to have a working knowledge in molecular biology and/or have completed graduate level molecular biology courses for best learning experience.

Instructors
This workshop is taught by subject matter experts from the National Institutes of Health and industry (MilliporeSigma).*

*This workshop is purely educational/training. FAES does not endorse any specific company/product; nor do not have any financial agreements/commission from MilliporeSigma.

Credit

Although no grades are given for courses, each participant will receive Continuing Education Units (CEUs) based on the number of contact hours. One CEU is equal to ten contact hours. Upon completion of this course each participant will receive a certificate, showing completion of the workshop and 3.5 CEUs.

Refund Policy

100% tuition refund for registrations cancelled 14 or more calendar days prior to the start of the workshop.

50% tuition refund for registrations cancelled between 4 to 13 calendar days prior to the start of the workshop.

No refund will be issued for registrations cancelled 3 calendar days or less prior to the start of the workshop.

Notification

All cancellations must be received in writing via email to Ms. Carline Coote at registrar@faes.org.

Cancellations received after 4:00 pm (ET) on business days or received on non-business days are time marked for the following business day.

Loading...

To Register Click on "Add to Cart"

Section Title
Genome Editing with CRISPR
Type
Online Synchronous
Days
M, T, W, Th, F
Time
9:00AM to 5:00PM
Dates
Mar 07, 2022 to Mar 11, 2022
Schedule and Location
Contact Hours
40.0
Location
  • ONLINE
Fee (Includes Tuition and Technology Fee)
Eligible Discounts Can Be Applied at Checkout $1,720.00
Required fields are indicated by .