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Course Description

This course provides a comprehensive overview of CRISPR-Cas9, a revolutionary gene-editing technology that has transformed biological research and holds immense potential for therapeutic applications. CRISPR-Cas9, adapted from a bacterial adaptive immune system, uses a programmable RNA guide to direct the Cas9 nuclease to a specific DNA sequence. This process allows for precise cleavage and modification of the target DNA.

Throughout this course, students will:

  • Master the fundamentals: Develop a deep understanding of the concepts, principles, and experimental procedures underlying CRISPR-Cas9 technology and tools.
  • Explore key delivery methods and applications: Gain practical skills in applying core CRISPR delivery methods, including lentiviral vectors, ribonucleotide protein complexes with cationic lipids, and plasmid-based approaches. A dedicated session will review the primary protocols I use routinely for CRISPR delivery commonly used for CRISPR screens and generating stable cell lines such as overexpressing Cas9. In addition, we will review current methods and applications of CRISPR, such as site-directed mutagenesis and genome-wide CRISPR screening.
  • Delve into genome-wide screening: Learn the methodology behind genome-wide CRISPR screens, a powerful tool for identifying genes involved in specific biological processes. This includes analyzing real data from Illumina sequencing using the online tool PinAPL-Py among others. You will compare your results to published data and discuss potential discrepancies and strategies for data validation.
  • Analyze data with bioinformatics: Become proficient in analyzing CRISPR screening data using bioinformatic tools. This course introduces user-friendly platforms, web-based application requiring no prior programming knowledge. While basic programming skills are not required, for those interested in exploring more advanced analysis, the course also covers MAGeCKflute (optional). I have extensive experience with both online platforms and command-line tools like MAGeCKflute, and I will guide you through the intricacies of each. No prior bioinformatics experience is required, as the course provides all necessary training.

Learner Outcomes

When you complete the course successfully, you will be able to:

  • Develop a thorough understanding of the principles underlying CRISPR gene-editing technology.
  • Analyze and compare different methods for introducing CRISPR components into cells, considering their advantages and limitations.
  • Identify and compare the various applications of CRISPR technology across diverse experimental contexts.
  • Utilize CRISPR-based genome-wide screens, in conjunction with bioinformatic analysis, to apply in scientific research.
  • Critically assess the current and potential future applications of CRISPR gene editing in treating human diseases, including associated challenges and ethical considerations.

Microcredential(s)

This course applies toward the Biomedical Sciences Curiosity interdisciplinary badge.

Textbook Information

There is no textbook for purchase required for this course.
 

Prerequisites

Basic understanding of molecular biology and cell biology.

Refund
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Scholarship and Funding

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Photo Release

By registering for this event, you agree to allow FAES to take photographs of you during the event and to use these photos for promotional purposes, including on our website, social media, and marketing materials, without further compensation. You understand that you have no right to review or approve the final use of these images.

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Section Title
Principles and Applications of CRISPR
Type
Online Asynchronous
Dates
Mar 25, 2026 to May 12, 2026
Total Cost (Includes $75 non-refundable technology fee per course when applicable)
Eligible Discounts Can Be Applied at Checkout (2 Credits) $775.00
Potential Discount(s)
Available for Academic Credit
2 Credit(s)
Instructor(s)
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