BIOC 062 - Image Processing and Analysis

Course Description

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Bioimaging studies are rapidly becoming more quantitative due to enhanced imaging technologies, improved analytical and computational tools, as well as increasingly more stringent scientific scrutiny for accuracy and reproducibility. However, there is a paucity of systematic and introductory surveys easily accessible to biologists when faced with a plethora of technical issues in digital image processing and analyses. The lack of clarity on this issue, compounded by debate over the methods abundant in the niche literature, frequently leads to further confusion for those whose primary expertise is not in digital image processing. Unfortunately, erroneous or misguided application of methods in biological imaging analyses is not uncommon, and this can lead to artificial inflation or suppression of biological significance – often unintentionally. The goal of this three-day hands-on workshop is to survey the fundamentals of how image pixel data can be used to extract biologically meaningful information.

The curriculum includes:
Day 1 (Image Processing)

Fundamentals of digital images
Introduction to digital image processing
Fourier transformation in image processing
Objection segmentation

Day 2 (Image Analysis)

Object-based colocalization
Quantitative image analysis – intensity measurement, pixel-based colocalization, ratiometric image analysis
Movement measurement
Object tracking

Day 3 (Machine learning)

Fundamental principles of machine learning
Machine learning tools for image classification and segmentation
Tools for image restoration and image translation
AI-facilitated event-driven microscopy
Importance of high-quality training data

Prerequisites

There are no prerequisites for this workshop.

Credit

Although no grades are given for courses, each participant will receive Continuing Education Units (CEUs) based on the number of contact hours. One CEU is equal to ten contact hours. Upon completion of the course each participant will receive a certificate, showing completion of the workshop and 2.1 CEUs.

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Photo Release

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